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一.產品名稱：Recombinant Human FGF-acidic

二.貨號Catalog Number:AF-100-17A

三.產地：美國Peprotech

四.同義Synonyms:Fibroblast Growth Factor-acidic, FGF-1, HBGF-1, ECGF-beta

五.描述Description:

FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant human FGF-acidic is a 16.0 kDa protein consisting of 141 amino acid residues. Manufactured using all non-animal reagents.

六.AA序列AA Sequence:

MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D

七.來源Source:E.coli

八.純度Purity:Greater than 95% by SDS-PAGE gel and HPLC analyses.

九.生物活性Biological Activity:

Assay #1: The ED₅₀ as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 10ng/ml corresponding to a specific activity of ≥ 1 x 10⁵ units/mg.

Assay #2: The ED₅₀ was determined by a cell proliferation assay using balb/c 3T3 cells is ≤ 0.5 ng/ml in the presence of 10 μg/ml heparin, corresponding to a specific activity of ≥ 2 x 10⁶ units/mg.

十.交叉性Cross Reactivity:

Cow, Mouse, Pig, Rat, Shark, Squid, Human